MEMBRA-CEL™ Dialysis Tubing and Membrane

MEMBRA-CEL Membrane is a hydrophilic material made from regenerated cellulose prepared using the viscose process. Typically this product is used in the laboratory for dialysis purposes. MEMBRA-CEL is available as tubing and sheets.

Technical Information
The molecular weight cut off of MEMBRA-CEL Tubing is 12,000-16,000 and the pore size is about 25 Angstroms. MEMBRA-CEL Membrane is available with molecular weight cut offs of 3500, 7000 and 14,000 with pore sizes ranging from 15 to 50 Angstroms. The pH stability range is 5 to 9 for both tubing and membrane.

Level of Contaminants • Sulphur compounds: Less than 0.3% • Heavy metals (atomic number greater than36): Less than 50 ppm

Chemical Compatibility • Cellulose has good compatibility with many salts such as CaCl2, (NH4)2SO4 and aqueous organic solvents commonly used in molecular biology/enzymology such as: isopropanol, ethanol and acetone.

Temperature Resistance
MEMBRA-CEL Tubing and Membrane can be boiled or sterilized by autoclave but should not be allowed to dry unless regylcerinated. Tubing that contains aqueous solutions can be frozen.

Microbial Resistance • Dialysis tubing and membrane are sensitive to cellulose activity, particularly when they are humidified. Growth of cellulolytic micro-organisms can be prevented by keeping the tubing in water containing benzoate, benzoic acid, formeldehyde or pentachlorophenol.

Protein Absorption • Protein absorption of MEMBRA-CEL Tubing and Membrane is less than 1 nanograms/gram of dry MEMBRA-CEL Tubing and Membrane.

Applications

Enzymology: • Desalting • Buffer changes • Trace inhibitor removal • Enzyme immobilization

Molecular biology: • Buffer changes

Handling